Temperature dependence of kinesin processivity
نویسندگان
چکیده
منابع مشابه
Kinesin Processivity
Conventional kinesin is a highly processive motor that can take Ͼ 100 steps along a microtubule before dissociating. Various lines of evidence have led to a model of hand over hand processive motion, in which the trailing head detaches and rebinds to the next open tubulin dimer site on the same protofilament, leading to an 8-nm movement of supports the hand over hand mechanism. Processivity is ...
متن کاملProcessivity of kinesin motility is enhanced on increasing temperature
Kinesin is a motor protein that processively moves step by step along a microtubule. To investigate the effects of temperature on run length, i.e., processivity of kinesin motility, we performed a single-molecular bead assay at temperature range of 20-40°C. An increase in the walking velocity of kinesin corresponded to the Arrhenius activation enthalpy of 48 kJ/mol, being consistent with the pr...
متن کاملKinesin processivity is gated by phosphate release.
Kinesin-1 is a dimeric motor protein, central to intracellular transport, that steps hand-over-hand toward the microtubule (MT) plus-end, hydrolyzing one ATP molecule per step. Its remarkable processivity is critical for ferrying cargo within the cell: over 100 successive steps are taken, on average, before dissociation from the MT. Despite considerable work, it is not understood which features...
متن کاملCommon general anesthetic propofol impairs kinesin processivity.
Propofol is the most widely used i.v. general anesthetic to induce and maintain anesthesia. It is now recognized that this small molecule influences ligand-gated channels, including the GABAA receptor and others. Specific propofol binding sites have been mapped using photoaffinity ligands and mutagenesis; however, their precise target interaction profiles fail to provide complete mechanistic un...
متن کاملMapping the Processivity Determinants of the Kinesin-3 Motor Domain.
Kinesin superfamily members play important roles in many diverse cellular processes, including cell motility, cell division, intracellular transport, and regulation of the microtubule cytoskeleton. How the properties of the family-defining motor domain of distinct kinesins are tailored to their different cellular roles remains largely unknown. Here, we employed molecular-dynamics simulations co...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2001
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.41.s194_4